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Immunohistochemistry for the novel markers glypican 3, PAX8, and p40 (ΔNp63) in squamous cell and urothelial carcinoma.

在鳞状细胞癌和尿路上皮癌中,新型标志物磷脂酰肌醇聚糖3,PAX8和P40(ΔNp63)的免疫组化研究。

Gailey MP,Bellizzi AM

Abstract

To examine squamous cell carcinomas (SCCs) from diverse anatomic sites and invasive urothelial carcinomas (UCs) for expression of the oncofetal antigen glypican 3 (GPC3), the paired box transcription factor PAX8, and the ΔN isoform of p63 (p40).
Immunohistochemistry for GPC3, PAX8, and p40 was performed on whole sections of 107 SCCs from 11 anatomic sites and 49 UCs; evaluation included extent and intensity of staining.
GPC3 was detected in 20% of SCCs and 12% of UCs and PAX8 in 3% of SCCs, limited to the uterine cervix, and 10% of UCs. p40 Was found in 99% of SCCs and 96% of UCs.
GPC3 expression is frequent in SCC/UC, awareness of which should guard against an incorrect diagnosis of hepatocellular carcinoma, while PAX8, limited in distribution, may have some use in suggesting a cervical or urothelial tract origin in a metastatic squamotransitional carcinoma of unknown primary. There is no drop-off in sensitivity for the diagnoses of SCC or UC with ΔNp63-specific immunohistochemistry, and if this performance can be extended to other applications, p40 may supplant the dominant "pan-p63" antibody clone.

摘要

目的:检测不同解剖部位来源的鳞状细胞癌(SCC)和侵袭性尿路上皮癌(UC)中癌胚抗原酰肌醇蛋白聚糖3(GPC3)、配对盒转录因子PAX8和p63的ΔN异构体(p40)。
方法:对107例来源于11个不同解剖部位的SCC和49例UC的所有切片进行GPC3,PAX8和P40免疫组化染色,并评估着色范围和着色强度。
结果:在20%的SCC和12%的UC中GPC3阳性;在3%的SCC和10%的UCS中PAX8阳性,且在SCC组中,只有子宫颈来源的PAX8阳性;在99%的SCC和96%的UC中p40阳性。
结论:GPC3经常表达在SCC/UC中,了解这点可以防止误诊为肝细胞性肝癌。而PAX8分布比较局限,在一些原发灶不明的转移性鳞状移行细胞癌中可能有提示宫颈或尿路上皮起源的作用。ΔNp63特异性免疫组织化学诊断SCC或UC的敏感性并不低,并且如果该作用可以扩展到其他研究项目中,p40可能取代主导的“pan-p63”的抗体克隆。

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