Microphthalmia transcription factor immunohistochemistry for FNA biopsy of ocular malignant melanoma.
眼部恶性黑色素瘤FNA活检组织小眼畸形转录因子（MiTF）免疫组织化学研究
Abstract
Microphthalmia transcription factor (MiTF) is a sensitive and specific immunohistochemistry (IHC) marker for malignant melanoma (MM) in surgical resections, but its utility in cytology specimens has not been extensively studied.
A search of the pathology database identified fine-needle aspiration biopsies signed out as MM or suspicious for MM (1998-2012). The final diagnosis and the IHC results were recorded, and all slides were re-reviewed. MiTF IHC (C5/D5 cocktail) was performed on alcohol-fixed, Papanicolaou-stained direct smears. Any amount of nuclear staining was considered positive. Staining was scored on a scale of 1 to 4 (1, 1%-24%; 2, 25%-49%; 3, 50%-74%; 4, 75%-100%).
Two hundred seventy-four cases were diagnosed as MM, and 24 were diagnosed as suspicious for MM. IHC staining was performed for 25% (69 of 274) of the MM cases and for 33% (8 of 24) of the cases suspicious for MM. MiTF IHC of Papanicolaou slides was performed for 37% (101 of 274) of the MM cases (positive, 82% [83 of 101]; negative, 4% [4 of 101]; noncontributory, 14% [14 of 101]) and for 33% (8 of 24) of the cases suspicious for MM (positive, 38% [3 of 8]; noncontributory, 62% [5 of 8]). The majority of positive staining was scored as 3 or 4. The sensitivity of MiTF on Papanicolaou-stained slides was 95%.
MiTF is a useful IHC stain for the evaluation of ocular MM, and it is effective when it is performed on alcohol-fixed, Papanicolaou-stained aspirate smears. Cancer (Cancer Cytopathol) 2015;123:394-400. © 2015 American Cancer Society.
A search of the pathology database identified fine-needle aspiration biopsies signed out as MM or suspicious for MM (1998-2012). The final diagnosis and the IHC results were recorded, and all slides were re-reviewed. MiTF IHC (C5/D5 cocktail) was performed on alcohol-fixed, Papanicolaou-stained direct smears. Any amount of nuclear staining was considered positive. Staining was scored on a scale of 1 to 4 (1, 1%-24%; 2, 25%-49%; 3, 50%-74%; 4, 75%-100%).
Two hundred seventy-four cases were diagnosed as MM, and 24 were diagnosed as suspicious for MM. IHC staining was performed for 25% (69 of 274) of the MM cases and for 33% (8 of 24) of the cases suspicious for MM. MiTF IHC of Papanicolaou slides was performed for 37% (101 of 274) of the MM cases (positive, 82% [83 of 101]; negative, 4% [4 of 101]; noncontributory, 14% [14 of 101]) and for 33% (8 of 24) of the cases suspicious for MM (positive, 38% [3 of 8]; noncontributory, 62% [5 of 8]). The majority of positive staining was scored as 3 or 4. The sensitivity of MiTF on Papanicolaou-stained slides was 95%.
MiTF is a useful IHC stain for the evaluation of ocular MM, and it is effective when it is performed on alcohol-fixed, Papanicolaou-stained aspirate smears. Cancer (Cancer Cytopathol) 2015;123:394-400. © 2015 American Cancer Society.
摘要
小眼畸形转录因子（MiTF）是外科切除恶性黑色素瘤（MM）中一种敏感而且特异的免疫组织化学（IHC）标记，但是其在细胞学样本中的应用尚未进行广泛的研究。
收集病理数据，从细针抽吸（FNA）活检组织中鉴别选出MM或疑似MM病例（1998-2012）。记录最终诊断及IHC结果，并且对所有的切片进行再次复审。在酒精固定、巴氏染色涂片上直接进行MiTF IHC染色（C5/D5鸡尾酒）。任何程度细胞核染色都认为是阳性。染色评分为1-4分（1： 1%-24%； 2：25%-49%； 3：50%-74%： 4： 75%-100%）。
274例诊断为MM，24例诊断为疑似MM。对25%（69/274）MM病例以及33%（8/24）疑似MM病例进行了IHC染色。37%MM病例（101/274）进行了巴氏切片MiTF IHC染色（82%阳性[83/101]，4%阴性[4/101]，14%无任何帮助[14/101]），33%疑似MM病例（8/24）进行了巴氏切片MiTF IHC染色（38%阳性[3/8]，62%无任何帮助[5/8]）。绝大多数阳性染色评分为3或4。在巴氏染色切片中MiTF的敏感性为95%。
MiTF是评估眼部MM的一种有用IHC标记，并且在酒精固定、巴氏染色的FNA涂片中进行IHC染色是有效的。
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