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Detection of BRAF Mutations on Direct Smears of Thyroid Fine-Needle Aspirates Through Cell Transfer Technique.

通过细胞转移技术在甲状腺细针穿刺的直接涂片中检测BRAF突变

Shi Q,Ibrahim A,Herbert K,Carvin M,Randolph M,Post KM,Curless K,Chen S,Cramer HM,Cheng L,Wu HH

Abstract

To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens.
Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases.
BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues.
PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.

摘要

目的是确定使用细胞转移技术(CTT)在甲状腺细针穿刺直接涂片(FNA)标本中检测BRAF分子突变的实用性。
在风干及乙醇固定的甲状腺FNA标本直接涂片中通过CTT获取组织,用聚合酶链技术(PCR)行BRAF分子检测,然后与相应的甲状腺切除标本、福尔马林固定、石蜡包埋(FFPE)组织相比较,共检测30例。
在29/30例风干CTT,27/30例乙醇固定CTT,和27/30例FFPE组织中成功进行了BRAF检测。结果显示,风干CTT、乙醇固定CTT和FFPE组织中分别有11、13和13例BRAF突变,有18、14和14例野生型。风干和乙醇固定标本CTT的一致率为96%,风干和FFPE标本CTT一致率为88%,乙醇固定和FFPE组织的CTT一致率为92%。
对甲状腺FNA标本直接涂片使用CTT并用PCR检测BRAF突变是可靠的。

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