We compare our experience using a new rapid qualitative immunoassay (RQI) test (platelet Pan Genera Detection, Verax, Worcester, MA) for bacterial screening of whole blood platelet (WBP) pools with our previous WBP bacterial screen, pH testing. All WBP pools were RQI tested at the time of issue. All RQI+ pools were cultured in an automated culture system, with subsequent bacterial identification if the culture was positive. During approximately 5.5 months, 7,733 WBP pools were RQI tested. There were 14 positive RQI tests; 12 WBP pools were sterile when cultured and considered false-positive RQI tests. One pool was positive for coagulase-negative Staphylococcus, while another was positive for group B Streptococcus. The specificity and positive predictive value of the RQI test were 99.85% and 14.3%, respectively. The specificity and positive predictive value of the RQI test were higher than pH testing, leading to less waste of sterile WBP pools.