Diagnosis of urinary tract infection (UTI) is primarily done by microbiologic culture, which is time-consuming and can produce false-positives and false-negatives. Flow cytometry allows for rapid screening of many samples and eliminates culturing. We analyzed the Sysmex UF-1000i (TOA Medical Electronics, Kobe, Japan) for accuracy in identifying RBCs and WBCs, casts, bacteria, and epithelia. We also evaluated its precision, linear estimation of results, carryover contamination rate, and anti-interference. UF-1000i agreement with manual counting was approximately 95% for RBCs and WBCs, epithelia, and casts. Its coefficient of variation for bacteria ranged from 4.7% to 15.2%. UF-1000i screening for UTIs exhibited great sensitivity (97%), specificity (79%), positive predictive value (70%), negative predictive value (99%), and accuracy (85%). The negative predictive value remained high even with complex UTI samples. The Sysmex UF-1000i shows great promise in excluding more than 50% of true-negative samples, improving detection efficiency, and reducing laboratory costs.