Comparison between cytospin and liquid-based cytology in urine specimens classified according to the Paris System for Reporting Urinary Cytology.
尿液细胞学标本巴黎报告系统用于离心涂片和液基薄层制片的比较
Abstract
The current study compared ThinPrep urinary cytology and conventional cytospin urinary cytology in the diagnosis of bladder cancer, applying the Paris System for Reporting Urinary Cytology.
Between January 2010 and December 2011, a total of 3659 urine samples were processed using conventional cytospin methods. Between January 2012 and December 2013, a total of 4186 urine cytological cases were analyzed using ThinPrep methods. In 131 cases (65 processed by conventional cytospin and 66 processed by ThinPrep), a subsequent biopsy was performed. The authors reclassified these cases according to the Paris System and an analysis between the 2 methods with regard to bladder biopsies was performed.
No significant differences were observed in terms of sensitivity and specificity between the 2 methods in cases with positive cytology for high-grade carcinoma. According to the Paris System, cases of atypical urothelial cells (AUC) and atypical urothelial cells suspicious for high-grade carcinoma (AUC-H) that were processed using cytospin did not correlate with urothelial carcinoma or with negative biopsies; conversely, the AUC cases processed using ThinPrep appeared to correlate with negative histological biopsies or low-grade urothelial carcinoma.
The results of the current study demonstrated that according to the Paris System, there were no significant differences in sensitivity or specificity for the diagnosis of high-grade urothelial carcinoma or AUC-H between the 2 methods. Cases of AUC should be easy to recognize using Thin Prep rather than cytospin and only AUCs diagnosed with ThinPrep were found to be statistically linked to negative cases for carcinoma or with low-grade urothelial carcinoma. Cancer Cytopathol 2016;124:519-23. © 2016 American Cancer Society.
Between January 2010 and December 2011, a total of 3659 urine samples were processed using conventional cytospin methods. Between January 2012 and December 2013, a total of 4186 urine cytological cases were analyzed using ThinPrep methods. In 131 cases (65 processed by conventional cytospin and 66 processed by ThinPrep), a subsequent biopsy was performed. The authors reclassified these cases according to the Paris System and an analysis between the 2 methods with regard to bladder biopsies was performed.
No significant differences were observed in terms of sensitivity and specificity between the 2 methods in cases with positive cytology for high-grade carcinoma. According to the Paris System, cases of atypical urothelial cells (AUC) and atypical urothelial cells suspicious for high-grade carcinoma (AUC-H) that were processed using cytospin did not correlate with urothelial carcinoma or with negative biopsies; conversely, the AUC cases processed using ThinPrep appeared to correlate with negative histological biopsies or low-grade urothelial carcinoma.
The results of the current study demonstrated that according to the Paris System, there were no significant differences in sensitivity or specificity for the diagnosis of high-grade urothelial carcinoma or AUC-H between the 2 methods. Cases of AUC should be easy to recognize using Thin Prep rather than cytospin and only AUCs diagnosed with ThinPrep were found to be statistically linked to negative cases for carcinoma or with low-grade urothelial carcinoma. Cancer Cytopathol 2016;124:519-23. © 2016 American Cancer Society.
摘要
该研究旨在根据尿液细胞学检查的巴黎分类报告系统比较膀胱癌诊断中离心涂片和液基薄层制片的细胞学检查结果。
收集2010年1月至2011 年12月间3659例尿液样本，进行常规离心涂片方法处理。收集2012年1月至2013年12月间4186例尿液标本，采用液基薄层制片术进行处理。随后进行了活检的有131例（65例常规处理和66例液基处理）；作者将这些做了膀胱活检的病例根据巴黎系统重新进行了分类。
这2种方法对于细胞学为高级别癌的病例来说，敏感性和特异性无显著差异。根据巴黎分类，细胞学诊断为非典型性尿路上皮细胞（AUC）和可疑高级别的非典型性尿路上皮细胞（AUC- H）在细胞离心涂片中为尿路上皮癌或阴性；反之，采用液基薄层制片的AUC病例则组织活检为阴性或低级别尿路上皮癌。
目前的研究表明，根据巴黎系统，两方法筛查高级别尿路上皮癌或AUC-H病例在敏感性和特异性方面没有显著差异。AUC病例使用液基薄层制片比离心涂片中更易识别，并且只有液基薄层制片中诊断的AUC病例与活检阴性或低级别尿路上皮癌有统计学联系。
我要评论
共0条评论