Comparative Immunohistochemical Analysis of IMP3, GLUT1, EMA, CD146, and Desmin for Distinguishing Malignant Mesothelioma From Reactive Mesothelial Cells.

对IMP3、GLUT1、EMA、CD146和Desmin的比较性免疫组织化学分析，以区分恶性间皮瘤和反应性间皮细胞。

Minato H,Kurose N,Fukushima M,Nojima T,Usuda K,Sagawa M,Sakuma T,Ooi A,Matsumoto I,Oda M,Arano Y,Shimizu J

阅读：732 American Journal of Clinical Pathology； 2014 January; 141 (1)

Abstract

Objectives: To identify useful biomarkers for differentiating between malignant mesothelioma (MM) and reactive mesothelial cells (RMCs). Methods: Formalin-fixed, paraffin-embedded (FFPE) tissues from 34 MM and 40 RMC samples were analyzed using immunohistochemistry, and the findings were compared. Results: Positive markers for MM included insulin-like growth factor 2 messenger RNA binding protein 3 (IMP3), glucose transporter 1 (GLUT1), epithelial membrane antigen (EMA), and CD146, which showed sensitivities of 94%, 85%, 79%, and 71% and specificities of 78%, 100%, 88%, and 98%, respectively. In sarcomatoid MM, EMA had significantly lower expression than did IMP3, GLUT1, and CD146 (P < .001). The areas under receiver operating characteristic curves were the highest for IMP3 (0.95), followed by GLUT1 (0.93). When the optimal cutoff points for IMP3 (30%) and GLUT1 (10%) were used, the sensitivity of IMP3 and GLUT1 for MM was 100%, and the specificity of both for MM was 95%. Conclusions: The combination of IMP3 and GLUT1 is most appropriate for distinguishing MM from RMC using FFPE sections.

摘要

目的：确定有助于区分恶性间皮瘤（MM）和反应性间皮细胞（RMC）的生物学标记。方法：用免疫组织化学法对经福尔马林固定石蜡包埋（FFPE）的34例MM和40例RMC组织进行分析，并比较结果。结果：MM阳性的标记包括胰岛素样生长因子2信使RNA结合蛋白3（IMP3）、葡萄糖载体1（GLUT1）、上皮膜抗原（EMA）和CD146，他们的敏感性分别为94%、85%、79%和71%，特异性分别为78%、100%、88%和98%。在肉瘤样MM中，EMA的表达低于IMP3、GLUT1和CD146（P<0.001）。imp3的受试者操作特性曲线下面积最高（0.95），其次为glut1（0.93）。如果将imp3和glut1的临界值分别定为30%和10%，二者对mm的敏感性可达100%，特异性可达95%。结论：在ffpe的切片中，为了区分mm与rmc，将imp3和glut1配伍使用是最合适的。

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